Position specific failures of flowcells

Rather than a general loss of quality for a whole sequencing lane or run sometimes partial failures occur. These can affect specific regions and cycles and can have knock on effects for the data generated

January 31, 2016 Illumina, FastQC

Incorrect encoding of Phred scores

Base call qualities in fastq and BAM format files are recorded using a text based encoding. Mistakes in the way this encoding is applied can lead to incorrect interpretation of base call accuracies in downstream analyses.

January 20, 2016 FastQC, QC Software

Sudden loss of base call quality

Sometimes a sequencing run can experience a sudden and lasting loss of base call quality across all sequences.

January 20, 2016 Illumina, FastQC, QC Software

Loss of base call accuracy with increasing sequencing cycles

Illumina based sequencing shows a loss of base call quality as the number of sequencing cycles performed increases.

January 20, 2016 Illumina, FastQC, QC Software