Illumina 2 colour chemistry can overcall high confidence G bases

With the introduction of the NextSeq system Illumina changed the way their image data was acquired so that instead of capturing 4 images per cycle they needed only 2. This speeds up image acquisition significantly but also introduces a problem where high quality calls for G bases can be made where there is actually no signal on the flowcell.

May 4, 2016 NextSeq, All Applications, Cutadapt, FastQC