Steven Wingett

Steven Wingett Posts written by Steven Wingett

Steven joined the Babraham Bioinformatics group in April 2011. He has an undergraduate degree in Molecular and Cellular Biology from the University of Bath and a DPhil in plant reproductive biology from the Department of Plant Sciences, University of Oxford. After completing his postgraduate degree, he worked in the information industry for several years.

Bioinformatics group, The Babraham Institute, Cambridge, UK

Illumina Patterned Flow Cells Generate Duplicated Sequences

The latest Illumina sequencers – such as the HiSeq X, HiSeq 3000 and HiSeq 4000 – use patterned flow cells to enable the discrimination between much more densely packed DNA clusters. While such technology substantially increases the number of reads generated per sequence run, this innovation may lead to an increased number of duplicates, thereby negating the improved yield and making subsequent data analysis potentially more difficult. Further investigation shows that these putative sequencing duplicates are generally in close two-dimensional proximity on a flow cell, which may provide an opportunity to develop bioinformatics solutions to identify and discard such artefacts.

March 2, 2017 HiSeq, All Applications, Bowtie2, HiCUP, Picard