FastQCFastQC Report
Tue 13 Aug 2024
dataset_2_Old_Mus_musculus_Bisulfite-Seq_2_val_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamedataset_2_Old_Mus_musculus_Bisulfite-Seq_2_val_2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences89543564
Total Bases7.8 Gbp
Sequences flagged as poor quality0
Sequence length20-100
%GC30

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGCATTCCTGCTGAACCGCTCTTCCG8578020.9579716974410355Illumina Paired End Adapter 2 (100% over 27bp)
CGGCATTCCTGCTGAACCGCTCTTCCGA1503840.16794506861486996Illumina Paired End Adapter 2 (100% over 28bp)
CGGCATTCCTGCTGAACCGC1291860.14427167540483424No Hit
CGGCATTCCTNCTGAACCGCTCTTCCG1111770.12415967718238242Illumina Paired End Adapter 2 (96% over 27bp)
TCGGCATTCCTGCTGAACCGCTCTTCCGA1008520.11262897688548561Illumina Paired End Adapter 2 (100% over 29bp)
CGGCATTCCTGCTGAACCGCT950270.10612376340079561Illumina Paired End Adapter 2 (100% over 21bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)