FastQCFastQC Report
Tue 13 Aug 2024
dataset_2_Old_Mus_musculus_Bisulfite-Seq_1_val_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamedataset_2_Old_Mus_musculus_Bisulfite-Seq_1_val_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences89543564
Total Bases7.8 Gbp
Sequences flagged as poor quality0
Sequence length20-100
%GC30

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCG6012030.6714083884353765Illumina Paired End Adapter 2 (100% over 27bp)
TCGGAAGAGCGGTTCAGCAGGAATGCCG1014400.11328564049561396Illumina Paired End Adapter 2 (100% over 28bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)